Table 4 Primers used for polymerase chain reaction (PCR) and DNA sequencing
Primer Name | Sequence | Gene | Purpose | Source |
|---|---|---|---|---|
STICHO-ITS-F-5′ | 5′-GGATCATTGAATCTATCAACAAC-3′ | ITS rDNA | PCR and Sequencing | [19] |
Dch-ITS-R | 5′-TGGTGGCCGAGCGGACGATT-3′ | ITS rDNA | PCR and Sequencing | This study |
ITS4-3′ | 5′-TCCTCCGCTTATTGATATGC-3′ | ITS rDNA | PCR and Sequencing | [59] |
Dch-5.8S-F-5′ | 5′-CGGATATCTTGGCTCCCGCAT-3′ | 5.8S Ribosomal region in ITS | Sequencing | This study |
Dch-5.8S-R-3′ | 5′-ACCGAAGTCTCGAGCGCAATA-3′ | 5.8S Ribosomal region in ITS | Sequencing | This study |
Dch-βactin-F | 5′-CAACACAGCGAGTGCCCTAT-3′ | β-actin protein | PCR and Sequencing | This study |
Dch-βactin-R | 5′-TGTACCGCACCTGTAAGCGC-3′ | β-actin protein | PCR and Sequencing | This study |